Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Antibacterial and Cytotoxic Activity of Compounds Isolated from Flourensia oolepis

doi: 10.1155/2015/912484

Figure Lengend Snippet: Anticancer effects of compounds 1 – 5 isolated from Flourensia oolepis extract toward sensitive and resistant leukemic cells.

Article Snippet: When the medium growth inhibitory concentration of 1 toward HEK293T (IC 50 = 67.0 μ M) and PBMC (IC 50 = 82.8 μ M) was compared to that of CCRF-CEM (IC 50 = 6.6 μ M), SIs of 10.1 and 12.5 were, respectively, observed, while in the case of CEM/ADR5000 (IC 50 9.9 μ M), the IC 50 ratios were 6.8 and 8.4, respectively.

Techniques: Isolation

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Antibacterial and Cytotoxic Activity of Compounds Isolated from Flourensia oolepis

doi: 10.1155/2015/912484

Figure Lengend Snippet: CCRF-CEM and K562 cells treated with 1% ethanol (controls (a) and (c), resp.) or with 1 at 2 × IC 50 ((b) and (d), resp.) for 72 h were stained with PI for cell cycle phase distribution analysis by flow cytometry. The percentages of cells in each phase, including sub-G1 (hypodiploid), are indicated.

Article Snippet: When the medium growth inhibitory concentration of 1 toward HEK293T (IC 50 = 67.0 μ M) and PBMC (IC 50 = 82.8 μ M) was compared to that of CCRF-CEM (IC 50 = 6.6 μ M), SIs of 10.1 and 12.5 were, respectively, observed, while in the case of CEM/ADR5000 (IC 50 9.9 μ M), the IC 50 ratios were 6.8 and 8.4, respectively.

Techniques: Staining, Flow Cytometry

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Antibacterial and Cytotoxic Activity of Compounds Isolated from Flourensia oolepis

doi: 10.1155/2015/912484

Figure Lengend Snippet: Effects of compound 1 on cell death process. CCRF-CEM and K562 cells were incubated for 72 h with 1% ethanol (controls (a) and (c), resp.) or with 2 × IC 50 of 1 ((b) and (d), resp.). Apoptotic effect of 1 was evaluated by flow cytometry analysis after double staining with Annexin V-APC and PI. Annexin V-APC intensity is represented on the x -axis and PI fluorescence intensity is represented on the y -axis.

Article Snippet: When the medium growth inhibitory concentration of 1 toward HEK293T (IC 50 = 67.0 μ M) and PBMC (IC 50 = 82.8 μ M) was compared to that of CCRF-CEM (IC 50 = 6.6 μ M), SIs of 10.1 and 12.5 were, respectively, observed, while in the case of CEM/ADR5000 (IC 50 9.9 μ M), the IC 50 ratios were 6.8 and 8.4, respectively.

Techniques: Incubation, Flow Cytometry, Double Staining, Fluorescence

Journal: Journal of Medicinal Chemistry

Article Title: Peptidomimetic α-Acyloxymethylketone Warheads with Six-Membered Lactam P1 Glutamine Mimic: SARS-CoV-2 3CL Protease Inhibition, Coronavirus Antiviral Activity, and in Vitro Biological Stability

doi: 10.1021/acs.jmedchem.1c00616

Figure Lengend Snippet: Human Cathepsin B and S Inhibition by Select Compounds

Article Snippet: This approach is consistent with that recently reported by Thorarensen and coworkers at Pfizer, who observed that an IC 50 determination after an incubation period was both insightful and an efficient method to assess trends for irreversible, covalent inhibitor programs.

Techniques: Inhibition